Soil respiration as affected by vegetation types in a semiarid region of China

There are variations in soil respiration across vegetation types; however, it is unclear which factors are mainly responsible for the variations. A field experiment was conducted in 2008 and 2009 in a semiarid region of China to investigate the daytime and monthly variation of soil respiration across vegetation types and to determine the factors controlling the variation. An automated portable soil carbon dioxide (CO₂) flux measurement system was used to measure the soil respiration in shrubland, grassland, fallow land, and cropland during the growing periods. The results showed that the relative daytime variation amplitude of soil respiration in the fallow land and cropland was as small as that of shrubland and grassland during July, but greater than that of shrubland and grassland during August and October. A hysteresis effect for the relationship between the daytime soil respiration and daytime soil temperature was observed for all four vegetation types. There was also a hysteresis effect for the relationship between the daytime soil respiration and daytime air temperature for the grassland. Over the study period, the monthly soil respiration rates of the fallow land and cropland were statistically comparable and significantly lower than those of the shrubland and grassland, with the exception of August, during which the monthly soil respiration of the cropland was as great as that of shrubland and grassland. The factors responsible for the monthly soil respiration variation across the vegetation types differed from month to month. In general, the soil temperature and soil water content were mainly responsible in August and September; however, the root biomass predominated in July and October. The results are valuable for accurately estimating regional carbon fluxes by considering the temporal variability of the soil respiration variation across vegetation types in the Loess Plateau of China.     

Binding of 2-nonanone and milk proteins in aqueous model systems

Interactions of the model flavor compound 2-nonanone with individual milk proteins, whey protein isolate (WPI), and sodium caseinate in aqueous solutions were investigated. A method to quantify the free 2-nonanone was developed using headspace solid-phase microextraction followed by gas chromatography with flame ionization detection. Binding constants (K) and numbers of binding sites (n) for 2-nonanone on the individual proteins were calculated. The 2-nonanone binding capacities decreased in the order bovine serum albumin > beta-lactoglobulin > alpha-lactalbumin > alpha s1-casein > beta-casein, and the binding to WPI was stronger than the binding to sodium caseinate. All proteins appeared to have one binding site for 2-nonanone per molecule of protein at the flavor concentrations investigated, except for bovine serum albumin, which possessed two classes of binding sites. The binding mechanism is believed to involve predominantly hydrophobic interactions.     

Peptides isolated from in vitro digests of milk enhance iron uptake by caco-2 cells

Milk proteins, during digestion, produce a range of biologically active peptides. Among those are peptides that may enhance iron absorption. The objective of this project was to investigate the effect of isolated milk peptides on iron uptake. Cow's milk, 0% fat, was subjected to a modified in vitro digestion process. The milk digest was further fractionated by gel filtration. All eluted fractions as well as beta-casein synthetic peptides (a tripeptide and a hexapeptide) were subsequently tested for effects on iron uptake with Caco-2 cell monolayers. Fractions of milk digests obtained through Sephadex G-25 gel filtration had a significant enhancing effect on iron uptake in Caco-2 cells compared to nonfractionated milk digests. Two fractions (P = 0) and the hexapeptide (P < 0.0001) enhanced iron uptake by up to 3-fold, whereas others and the tripeptide had no effect. These results suggest that selected peptides produced during the in vitro digestion of milk may enhance iron absorption; however, it remains to be demonstrated whether this effect may be nutritionally significant.     

基于植被覆盖率的农村居民点整理潜力估算及实证

为合理估算农村居民点整理潜力,该文提出基于植被覆盖率的农村居民点整理潜力测算新思路。借助植被覆盖率对农村居民点斑块进行分类,通过分析抽样农村居民点斑块的植被覆盖与内部用地结构得到整理潜力修正系数,从而建立基于植被覆盖率修正的农村居民点整理潜力估算模型,并以北京市门头沟区为例展开实证研究。研究结果表明,门头沟区无整理潜力的农村居民点占大多数,具有整理潜力的农村居民点主要集中在经济相对落后的乡镇,并且这部分农村居民点多远离主干道;山区具有中、高整理潜力的农村居民点略多于平原、浅山区,而低整理潜力的农村居民点明显多于平原、浅山区;门头沟区规划整理的农村居民点内部包含有大量农用地,其中建设用地比例只占63.19%～71.54%,但是,第二次土地调查将整个农村居民点都作为建设用地调绘;因而,一些不考虑植被覆盖修正的农村居民点整理潜力测算方法夸大了整理潜力,放大了城乡建设用地增减挂钩的效果。该文为进一步合理估算农村居民点整理理论潜力提供参考。     

茶树油清除豇豆农药残留的效果

为研究茶树油清除果蔬农药残留的效果,该试验选取豇豆为供试材料,以不同浓度的茶树油和水溶性茶树油等清洗处理,利用气相色谱和气相色谱-质谱联用检测豇豆内有机磷类、拟除虫菊酯类和氨基甲酸酯类的农药残留量,计算农药清除率。供试7种农药中,水胺硫磷、马拉硫磷、氧乐果、三唑磷、毒死蜱、氯氰菊酯和速灭威在豇豆中的初始浓度分别为:20.395、1.690、6.524、10.719、0.160、12.104和23.057mg/kg。茶树油处理后检测结果表明,茶树油具有清除残留在豇豆中农药的能力,清除效果随茶树油浓度增加而增强;清除有机磷类农药效果较拟除虫菊酯类和氨基甲酸酯类农药明显。茶树油比去离子水、市售果蔬农残清洗剂清除农药残留效果显著,同时,相同浓度的水溶性茶树油比相应茶树油清除农药残留能力强。0.8%水溶性茶树油清除效果最佳,清除率分别为水胺硫磷80.48%,马拉硫磷94.54%,三唑磷82.79%,毒死蜱84.58%,氧乐果72.20%,氯氰菊酯80.51%,速灭威72.21%。通过研究结果可知,茶树油可作为有开发前景的果蔬清除剂。     

便携式作物生长监测诊断仪的设计与试验

为了解决当前作物生长信息实时、快速、无损测量手段瓶颈问题,研发了一种基于多光谱传感器的便携式作物生长监测诊断仪。该仪器由多光谱传感器系统、处理器系统及附属机构组成,能实时无损地获取作物叶层氮含量、叶层氮积累量、叶面积指数、生物量等主要生长指标。对水稻的试验结果表明,便携式作物生长监测诊断仪测得的DVI值与水稻叶层氮含量、氮积累量、叶面积指数、叶干重的决定系数R2分别为0.711,0.8231,0.7698,0.7212。该仪器结构简单,集成度高,性价比好,携带方便,易于田间操作。     

新型杀螨剂F1050在不同类型土壤中的降解研究

农药作为外来化合物，必然参与环境中物质循环和代谢，并影响环境的能量和物质循环。近年来，越来越多的新化合物进入农业环境，给农业生态系统带来了极大的压力，同时给人们提出了如何消除这种压力的问题。农药的迁移、吸附、代谢、富集等环境行为及其所产生的生态效应，尤其是有机污染物在环境中的降解问题备受关注。     

Evidence of N<Subscript>2</Subscript>O emission and gaseous nitrogen losses through nitrification-denitrification induced by rice plants (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.)

A greenhouse experiment was conducted with a specially designed apparatus consisting of an upper and lower chamber where the treatment with rice was carried out (treatment 1). The apparatus also had a single chamber where treatment 2, without rice plants, was carried out. The scope of this study was to elucidate the influence of rice plant growth on gaseous N losses as N₂ and N₂O produced by nitrification-denitrification in a flooded soil fertilized with (NH₄)₂SO₄ (with 56.50 atom% ¹⁵N). Gas samples were withdrawn weekly and analyzed for (N₂ + N₂O)-¹⁵N losses by mass spectrometer and for N₂O by gas chromatograph. The gaseous (N₂ + N₂O)-¹⁵N losses of the treatment with rice plants were significantly (P =0.01) higher than those of the treatment without rice plants, as were the amounts of N₂O emitted. Rice plants facilitate the efflux of N₂ and N₂O from soil to atmosphere, as about half of the total gaseous ¹⁵N loss as N₂ and N₂O was found in the upper chamber. The proportion of N₂O-¹⁵N to (N₂ + N₂O)-¹⁵N in the upper chamber was 10.56%, much higher than that of the lower chamber in treatment 1 and the headspace of treatment 2.     

Genetic diversity of winter wheat in Shaanxi province, China, and other common wheat germplasm pools

Cultivated Chinese wheat germplasm has been a valuable genetic resourcein international plant breeding. Landraces endemic to China are a geneticresource that is distinct from other wheat germplasm. Patterns of geneticdiversity among cultivated Chinese accessions and relationship to othergermplasm pools are unknown, despite the proven value and potential novelty. Theobjective of this work was to determine the level of genetic diversity withinimproved Chinese germplasm in the context of several other wheat germplasmpools. We analyzed a set of improved accessions cultivated from the 1940s to the1990s in Shaanxi province, China, using six simple sequence repeat (SSR) primerpairs and 30 restriction fragment length polymorphism - probe enzymecombinations (RFLP-PEC) previously used to characterize 21 geographically basedgermplasm pools. Shaanxi germplasm consists of three groups based on foreignintroductions from Italy, Australia, Denmark, and Russia. There was a decreasein genetic diversity among Shaanxi accessions cultivated in the 1970s and 1980sto the 1990s, and accession classifications based on primary decade ofcultivation were found to be significantly undifferentiated. The analysis of themean genetic distance among 22 geographically based pools of germplasm suggestsseveral regions are significantly undifferentiated. A vast majority of the totalamount of variation was found within pools; therefore, pools appear to belargely differentiated based on small differences in band relative frequency andfew if any unique bands. Previous studies have identified some Chinese landracepools as morphologically and genetically unique. The Shaanxi pool does not havethe same unique morphological or genetic features, nor is it more similar to thelandrace pools than other improved germplasm pools.     

中熟大果型番茄皖粉3号的选育

皖粉3号系以美国材料中经多代系统育成的优良自交系92-8-28为母本，以本所育成的优良自交系92-15-9为父本配制而成的番茄一代杂种。属无限生长类型，中熟，抗多种病害(TMV、灰霉病、叶霉病、早疫病)，温光适应范围广。果实高圆形，粉红色，果面光滑，果皮厚，耐贮运，品质佳，可溶性固形物含量5.5%～6.2%，甜酸适度，单果质量350g左右，单产可达11.25万kg·hm-2。适宜保护地和春夏露地高架栽培。目前已在安徽、江苏、河南、江西、山东、陕西、河北、新疆等地示范推广。